![ape sequence analysis ape sequence analysis](https://i.ytimg.com/vi/bU_yoYj-ngs/maxresdefault.jpg)
equi to current antimicrobial treatment, new tools that can provide a fast and accurate diagnosis of the disease and antimicrobial resistance profile are needed. equi is an important veterinary pathogen that takes the lives of many foals every year. Here the authors introduce QUEEN, a framework to describe and share DNA materials and construction protocols. DNA constructs and their annotated sequence maps have been rapidly accumulating with the advancement of DNA cloning, synthesis, and assembly methods. We propose QUEEN as a solution to start significantly advancing the material and protocol sharing of DNA resources. QUEEN-generated GenBank files are compatible with existing DNA repository services and software.
#APE SEQUENCE ANALYSIS CODE#
A GenBank file generated by QUEEN can regenerate the process code such that it perfectly clones itself and bequeaths the same process code to its successive GenBank files, recycling its partial DNA resources.
![ape sequence analysis ape sequence analysis](https://scitechdaily.com/images/Great-Ape-Y-Chromosome-Evolution-777x583.png)
QUEEN enables the flexible design of new DNA by using existing DNA material resource files and recording its construction process in an output file (GenBank file format). Here, we report a framework QUEEN (framework to generate quinable and efficiently editable nucleotide sequence resources) to resolve these issues and accelerate the building of DNA. Furthermore, the use of previously developed DNA materials and building protocols is usually not appropriately credited. However, as commonly seen in the life sciences, no framework exists to describe reproducible DNA construction processes. Such resources have also been utilized in designing and building new DNA materials. Taken together, we provide a versatile delivery platform for single base to multi-gene level genome interventions, addressing the currently unmet need for a powerful delivery system accommodating current and future CRISPR technologies without the burden of limited cargo capacity.ĭNA constructs and their annotated sequence maps have been rapidly accumulating with the advancement of DNA cloning, synthesis, and assembly methods. We demonstrate single baculovirus vectored delivery of single and multiplexed prime-editing toolkits, achieving up to 100% cleavage-free DNA search-and-replace interventions without detectable indels. We use our approach to rescue wild-type podocin expression in steroid-resistant nephrotic syndrome (SRNS) patient derived podocytes. By encoding Cas9, sgRNA and Donor DNAs on a single, rapidly assembled baculoviral vector, we achieve with up to 30% efficacy whole-exon replacement in the intronic β-actin (ACTB) locus, including site-specific docking of very large DNA payloads. Here we exploit the unmatched heterologous DNA cargo capacity of baculovirus to resolve this bottleneck in human cells. This challenge represents a major roadblock to genome engineering applications. CRISPR-based precise gene-editing requires simultaneous delivery of multiple components into living cells, rapidly exceeding the cargo capacity of traditional viral vector systems.